• Inspect your bed. Bed bugs hide in and around upholstery and fabric, usually within 15 feet of the bed. To check your hotel room bed before you recline, pull up the linens at a top corner of the bed, then pull off the corner of the mattress pad, and examine the mattress seams. Look for red or brownish specks or black, near-microscopic fecal material. If you don’t see signs here, chances are your bed is bug-free.
• Don’t forget couches, chairs, headboards. Just as you checked your mattress, check the corner seams of any upholstered furnishings. Another common hiding place is the vinyl-upholstered headboards bolted to the wall in many hotel rooms: look carefully at the seam between headboard and wall.
• Two floors of separation. Following your inspection, should you need to request another room, be sure to ask for one at least two floors away from the original. Bedbugs are known to travel hallways from one room to another and to travel inside walls via wiring conduit.
For more tips on avoiding bed bugs on your summer vacation, check out this article from Health.com.